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Angiopoietin-2 and VEGF165 ELISA kits join the Diaclone catalogue !!

Angiopoietin-2 (Ang-2) is a key regulator of angiogenesis that exerts context-dependent effects on endothelial cells (ECs). Ang-2 binds the endothelial-specific receptor tyrosine kinase 2 (Tie2) and acts as a negative regulator of Ang-1/Tie2 signaling during angiogenesis. Serum levels of Ang-2 expression are associated with the growth of multiple myeloma, angiogenesis, and overall survival in oral squamous cell carcinoma. Circulating Ang-2 is a marker for early cardiovascular disease in children on chronic dialysis.

Vascular endothelial growth factor (VEGF), originally known as vascular permeability factor (VPF),is a signal protein produced by cells that stimulates vasculogenesis and angiogenesis.

When VEGF is overexpressed, it can contribute to disease. Solid cancers cannot grow beyond a limited size without an adequate blood supply; cancers that can express VEGF are able to grow and metastasise. Overexpression of VEGF can cause vascular disease in the retina of the eye and other parts of the body.

Some authors have suggested that interaction between VEGF and Ang-2 may play a critical role in tumor angiogenesis in Hepatocellular carcinoma.

 

Why Diaclone ELISA ?

Quality

  • High sensitivity & dynamic range – for detection of low pg/mL levels of target in various samples (Serum, Plasma, cell supernatant …..)
  • High Specificity & Precision – detect both natural and recombinant proteins with no cross reactivity to related proteins
  • Reliability & Consistency – robust validation and manufactured to ISO9001:2008 quality standards

 

Flexible

  • ELISA reagents available in a number of formats from pre-coated kits for easy analysis and increased throughput to matched antibody pairs, a great tool for developing research
  • Various ELISA kits sizes 1x96, 2x96 and 1x48 discover Diaclone cost effective trial size
  • Pre-coated plates with removable strip
  • Custom bulk production

 

Trusted

  • Diaclone antibodies and Immunoassays are utilised by key companies across the life-science industry
  • Experienced & responsive technical support
  • Control of own high quality antibody development

 

Angiopoietin-2 and VEGF165 ELISA kit Specifications

Specifications

Human Ang-2 ELISA kit

Human VEGF165 ELISA kit

Specificity

Recognizes natural and recombinant Angiopoietin-2

Recognizes human VEGF165 isoforms

Range

125 – 4000 pg/mL

31.25 – 1000 pg/mL

Sensitivity

25 pg/mL

15.9 pg/mL

Incubation

3h45

3h45

Sample types

Serum, Plasma, Cell Culture Supernatant

Serum, Plasma, Cell Culture Supernatant

Sample size

50 µl

50 µl

Cross Reactivity

No cross reactivity with VEGF-A, GM-CSF, IL-34, IL-29 IFNg, IL-2, IL-10, CD138, MICB, MCP-1

No cross reaction with VEGF-121, VEGF-145, VEGF-B, VEGF-C, VEGF-D, VEGF-R1, Ang-2.
Sho
ws cross reactivity with VEGF-189 isoform

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Formats and Related Products

Product

Format

Cat. No.

Ang-2

Human Ang-2 ELISA kit

1x48 Discovery with pre-coated plates

873.080.048

Human Ang-2 ELISA kit

1x96 with pre-coated plates

873.080.096

Human Ang-2 ELISA kit

2x96 with pre-coated plates

873.080.192

VEGF165

Human VEGF165 ELISA kit

1x48 Discovery with pre-coated plates

873.070.048

Human VEGF165 ELISA kit

1x96 with pre-coated plates

873.070.096

Human VEGF165 ELISA kit

2x96 with pre-coated plates

873.070.192

Related Products

Anti Human Ang-2 monoclonal antibody

Azide free clone B-C57

855.960.000

Anti Human Ang-2 monoclonal antibody

Azide free clone B-G60

855.970.000

Anti-Human VEGF165 monoclonal antibody

Azide free clone B-G55

855.920.005

Anti-Human VEGF165 monoclonal antibody

Azide free clone B-F55

855.930.005

Human VEGF-A ELISA Kit

1 x 96 With pre-coated plates

650.080.096

Human VEGF-A ELISA Kit

2 x 96 With pre-coated plates

650.080.192

Human VEGF-R1 ELISA kit

1 x 96 With pre-coated plates

650.030.096

Human VEGF-R1 ELISA kit

2 x 96 With pre-coated plates

650.030.192

Mouse VEGF-A ELISA kit

1 x 96 With pre-coated plates

660.140.096

Mouse VEGF-A ELISA kit

2 x 96 With pre-coated plates

660.140.192

Rat VEGF-C ELISA kit

1 x 96 With pre-coated plates

670.110.096

Rat VEGF-C ELISA kit

2 x 96 With pre-coated plates

670.110.192